Strategies to increase local T3 availability in liver

Prof. Dr. Josef KöhrleDr. Eva K. Wirth

T3 is a key regulator of hepatic lipid metabolism. Hypothyroidism has been linked to non-alcoholic (obesity-/ diabetes-associated) hepatosteatosis and its sequelae. We aim to elevate local T3 concentrations by steering activities of T3-degrading deiodinase isozymes in hepatocytes by using selective deiodinase (DIO) isozyme inhibitors. Local T3 effects will be monitored by T3-sensitive reporter constructs expressed in THAI mouse-derived primary hepatocyte cultures and hiPSC-derived hepatocyte cell lines.


Project Team Members

Caroline Frädrich PhD candidate (Team Köhrle)   This email address is being protected from spambots. You need JavaScript enabled to view it.
Rajas Sane PhD candidate (Team Köhrle, Wirth)   Mail
Joshua Seifert PhD candidate (Team Köhrle, Wirth)    
Anja Fischbach Lab Technician (Team Köhrle)    

Further Information

Homepage Team Köhrle

Homepage Team Wirth

Selected Publications

3,5-T2 A Janus-Faced Thyroid Hormone Metabolite Exerts Both Canonical T3-Mimetic Endocrine And Intracrine Hepatic Action.
Köhrle J, Lehmphul I, Pietzner M, Renko K, Rijntjes E, Richards K, Anselmo J, Danielsen M, Jonklaas J.
2020. Front Endocrinol (Lausanne) 10:787. doi: 10.3389/fendo.2019.00787

A thyroid hormone-independent molecular fingerprint of 3,5-diiodothyronine suggests a strong relation with coffee metabolism in humans.
Pietzner M, Köhrle J, Lehmphul I, Budde K, Kastenmüller G, Brabant G, Völzke H, Artati A,  Adamski J, Völker U, Nauck M, Friedrich N, Homuth G.
2019. Thyroid 29(12):1743-1754. doi: 10.1089/thy.2018.0549

3-iodothyronamine – a thyroid hormone metabolite with distinct target profiles and mode of action.
Köhrle JBiebermann H.
2019. Endoc Rev 40(2):602-630. doi: 10.1210/er.2018-00182

A combined LC-MS/MS and LC-MS3 multi-method for the quantification of iodothyronines in human blood serum.
Richards KH, Monk R, Renko K, Rathmann D, Rijntjes E, Köhrle J.
2019. Anal Bioanal Chem 411(21):5605-5616. doi: 10.1007/s00216-019-01941-9

3,5-Diiodo-L-thyronine (3,5-T2) exerts thyromimetic effects on hypothalamus-pituitary-thyroid axis, body composition, and energy metabolism in male diet-induced obese mice.
Jonas W, Lietzow J, Wohlgemuth F, Hoefig CS, Wiedmer P, Schweizer U, Köhrle J*, Schurmann A*. *equal contribution
2015. Endocrinology 156(1):389-99. doi: 10.1210/en.2014-1604